Cooperative Institute for Research in Environmental Sciences

Science Rendezvous > 2009 Posters
The presenter is listed in italics. All poster presentations are the property of their respective authors. Please contact the authors for permission to use images, information, or ideas.

Carbon Dioxide Assisted Nebulization with a Bubble Dryer© (CAN-BD) Processing of Pharmaceuticals for Inhalable Needle-Free Delivery

JESSICA L. BURGER, ¹ STEPHEN P. CAPE, ¹ CHAD S. BRAUN, ¹DAVID H. MCADAMS, ¹ JESSICA A. BEST, ¹ PRADNYA BHAGWAT,² PANKAJ PATHAK,² LIA G. REBITS, ² ROBERT E. SIEVERS, ¹,²

¹Center for Pharmaceutical Biotechnology, Department of Chemistry and Biochemistry, and CIRES, 214 UCB, University of Colorado, Boulder, CO 80309, USA. Phone: (303) 492-7943 fax: (303) 492-1414
²AKTIV-DRY, LLC, 6060 Spine Road, Boulder, CO 80301, USA. Phone: (303) 350-3060 Fax: (303) 492-1414

Carbon Dioxide Assisted Nebulization with a Bubble Dryer® (CAN-BD) dries and micronizes products at near ambient conditions, giving good retention of bioactivity and proving to be less costly and time-consuming than lyophilization. Processing allows particles to be made in the 3-5 µm size range, which is desirable for lung delivery, without destroying biological activity. Our group has been developing stabile micronized pharmaceutical powders for interpulmonary delivery. The CAN-BD (Carbon Dioxide Assisted Nebulization with Bubble Drying ) process has been shown to successfully produce stabile dry powders of vaccines and antibiotics. In response to the Grand Challenge in Global Health Initiative #3, we have been focused on developing an inhalable needle-free live attenuated measles virus vaccine for use in developing countries. Measles was chosen for the vaccine of choice because it is the number one vaccine preventable killer of children worldwide. Powders are analyzed using varying techniques including x-ray diffraction, scanning electron microscopy, Andersen cascade impaction, differential scanning calorimetery, Karl Fischer titration and viral plaque assay. CAN-BD has been used to produce powder of live attenuated measles virus vaccine with characteristics desirable for lung delivery. The powders retain viral activity forming and drying the microparticles by CAN-BD and have passed the WHO stability test for one week at 37 ?C. Of current interest are formulations containing myo-inositol as they retain high viral activity and have low initial water content.