DAURE Microscopy

This page contains electron microscopy images acquired by Esther Coz of CIEMAT/IDAEA-CSIC at RJ Lee Group, Inc.

The images presented above have been acquired with two different high resolution Scanning Electron Microscopes (FEI Sirion400 and Hitachi S5500). Background has been automatically substracted from images to enhance the morphology, but no post processing has been applied on the particles after acquisition. The different relative content of each of the groups (and subgroups) presented below has been quantified and its variations at different times of the day have been analyzed together with the morphological descriptors of each group with a fully automated SEM (PSEM 500). The data is still under review/analysis. Any commentaries, suggestions or corrections (if any mistake is found) are welcome. Please, email esther.coz@ciemat.es.

DAURE participants are allowed to use the pictures for meetings and presentations with proper acknowledgment. Please, contact Esther Coz in case of publishing to check the image has not been already used.

Images from the BCN site

Primary Biogenic Organic Particles

Despite not being an important group in abundance, the microscopy analysis of PM2.5 samples from BCN site revealed a very rich diversity of this particle type. Several families of spores, vegetative and insect detritus and bacteria have been identified with sizes from 300 nm up to 4 µm. Some of them are frequently mixed with other anthropogenic aerosols.

	Particle BCN-1 Fungi spore Sampling: 02/25/09, 7- 9 pm Size = 3.3 µm Composition: Major components: C, O; Minor components: S, Cl, Ca Notes: Hiliferous appendix on the lower right, smooth ornament.
	Particle BCN-2 Non classified Sampling: 02/28/09, 5.30-7.10 pm Size = 410 nm Composition: Major components: C, O; Minor components: S, Cl Notes: Morphology with circular aperture and ornament that reminds a fungi spore.
	Particle BCN-3 Vegetative detritus Sampling: 02/26/09, 7.40-8.20 pm Size = 440 nm Composition: Major components: C, O; Minor components: Al, Si (not always) Notes: The morphology reminds a vegetative tissue in which some volatile material was initially entrapped (see the open cut).
	Particles BCN-4 & BCN-5 Fungi spores (possible) Sampling: 02/25/09, 7-9 pm Size = 420 nm Composition: Major components: C, O; Minor components: S, Si, Cl, Na, K Notes: Different morphologies of possible fungi spores with similar size and elemental composition. The one on the lower right has golf ball texture and seems to have volatile contain. Both of them are very abundant in the samples and frequently found mixed with soot agglomerates.

Heavy Metal Oxides

Frequently found in the PM2.5 samples collected during traffic peak hours with sizes from 800 nm up to 5 µm. Some of them are frequently mixed with mineral particles. The most abundant are iron oxides (frequently linked to minor contents of titanium and zinc).

	Particle BCN-6 Iron oxide Sampling: 02/25/09, 7-9 pm Size = 1.9 µm Composition: Major components: Fe2O3; Minor components: Si, S, Ti, Mg, Cl, Na Notes: Agglomerate structure and associated minor elements typical from vehicle emissions.
	Particle BCN-7 Iron-zinc oxide Sampling: 02/26/09, 11-12 am Size = 1 µm Composition: Fe2O4Zn; Minor components: Mn, Cr, Ca, Mg, S, Si, Al Notes: Spherical morphology frequently associated to industrial combustion processes.
	Particle BCN-8 Iron oxide Sampling: 02/25/09, 7-9 pm Size = 3 µm Composition: Fe2O3; Minor components: Si, S, Cu, Cl, Mg, Al, Ca Notes: Associated minor elements indicate possible anthropogenic origin.

Mineral Particles

	Particle BCN-9 Spherical AluminoSilicate (SAS particle) Sampling: 02/28/09, 8.45 - 9.25 pm Size = 800 nm Composition: SiO2, Al2O3; Minor components: Ca, S, Na, Fe Notes: Spherical aluminosilicate related to coal fired power plant emissions. Also denoted as fly-ash. Frequently found as single particles (not agglomerated) with a wide size range from several nanometers to several micrometers.
	Particle BCN-10 Calcium carbonate (calcite) Sampling: 02/25/09, 7-9 pm Size = 1.6 µm Composition: CaCO3
	Particle BCN-11 Quartz Sampling: 02/25/09, 7-9 pm Size = 1.7 µm Composition: SiO2 Notes: Image taken at 2 kV (left) and 15 kV (right) allows to compare how different particles may look if changed any operation value.
	Particle BCN-12 Calcium sulfate (gypsum) Sampling: 02/25/09, 7-9 pm Size = 1.1 µm Composition: CaSO4-2(H2O) Notes: Tabular crystal of calcium sulfate(probably gypsum).

Soot-like Agglomerates

Soot-like is the most abundant particle structure for all the samples independently of the day and time of sampling. Larger percentage of "fresh" type (BCN-13) are found on samples associated to traffic peak hours, when the opposite trend (larger abundance of "aged" type, BCN-14) occurs for samples collected during the rest of the sampling times. These types of structures are generally linked to diesel emissions. However, other type of aerosols, such as certain biogenic organic compounds, can be also linked to this type of structures.

	Particle BCN-13 Fresh soot-like agglomerate Size = 290 nm Composition: Major components: C, O; Minor components: S, Si, Na Notes: Chain-like structure of agglomerates generally indicates the aerosol is fresh. Size of the primitive particles ranges from 40-60 nm. The agglomerate is coated with a sulfate (possible organic/sulfate mixture) layer.
	Particle BCN-14 Aged soot-like agglomerate Size = 390 nm Composition: Major components: C, O; Minor components: S, Si, Na Notes: Sponge structure indicates aerosol aging. The coating thickness in this type of structues strongly varies with the time of the day due to the condensation of organics on the surface.

Other Carbonaceous Particles

Examples of some other carbonaceous particles (probably organic aerosols) found in the samples are presented next.

	Particle BCN-15 Other carbonaceous particles Size = 350 nm Composition: Major components: C, O Notes:
	Particle BCN-16 Other carbonaceous particles Size = 530 nm Composition: Major components: C, O Notes:

Sulfates

	Particle BCN-17 Sulfate Size = 510 nm Composition: Major components: S, O Notes: Probably ammonium sulfate or bisulfate
	Particle BCN-18 Sulfate Size = 215 nm (360 nm, sulfate + soot-like) Composition: Major components: S, O Notes: Bubble shaped ammonium sulfate linked to a soot-like structrure typical from diesel emissions.

Images from the MSY site

Upcoming...

Acknowledgements

The electron microscopy images contained in this page have been acquired by Esther Coz of CIEMAT/IDAEA-CSIC at RJ Lee Group, Inc.

The work has been partially funded by the following projects of the Spanish Ministry of Science and Innovation:
- GRACCIE Project, CSD2007:0067
- Acción Complementaria CGL2008- 02817-E/CLI
- Project CGL2007-64117/CLI