Difference between revisions of "CHEM-5181"
From Jimenez Group Wiki
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=== Ionization Sources === | === Ionization Sources === | ||
− | * Introduction and Electron Ionization | + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Lect/I1_Ionization_I_EI.pdf Introduction and Electron Ionization] |
** [http://www.vias.org/simulations/simusoft_ionoptics.html Ion Optics Simulator] | ** [http://www.vias.org/simulations/simusoft_ionoptics.html Ion Optics Simulator] | ||
** ''Prop: EI ionizer, filaments, magnets'' | ** ''Prop: EI ionizer, filaments, magnets'' | ||
− | * Electrospray and its Ion Chemistry (from Invited Lecturer Dr. Shuji Kato) | + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Lect/ESI_lecture_Oct26_2010.pdf Electrospray and its Ion Chemistry (from Invited Lecturer Dr. Shuji Kato)] |
** ''Prop: ESI Source'' | ** ''Prop: ESI Source'' | ||
− | * Chemical Ionization | + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Lect/I3_CI.pdf Chemical Ionization] |
** [http://www.ptrms.com PTRMS Commercial Instrument] | ** [http://www.ptrms.com PTRMS Commercial Instrument] | ||
** [http://www.syft.com SYFT Commercial Instrument] | ** [http://www.syft.com SYFT Commercial Instrument] | ||
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* [http://cires.colorado.edu/jimenez/CHEM-5181/Lect/L_dePuy_Flowing_Afterglow.pdf Invited Lecture: the Flowing Afterglow] by Prof. Chuck dePuy | * [http://cires.colorado.edu/jimenez/CHEM-5181/Lect/L_dePuy_Flowing_Afterglow.pdf Invited Lecture: the Flowing Afterglow] by Prof. Chuck dePuy | ||
− | * Ionization IV: Bombardment, MALDI and Elemental | + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Lect/I4_Ionization_Others.pdf Ionization IV: Bombardment, MALDI and Elemental] |
** [http://instaar.colorado.edu/~marchitt//lab.html ICP-MS Facility at CU-Boulder] | ** [http://instaar.colorado.edu/~marchitt//lab.html ICP-MS Facility at CU-Boulder] | ||
** ''Prop: MALDI plate'' | ** ''Prop: MALDI plate'' |
Revision as of 17:15, 7 December 2010
This is the new home for the CU-Boulder Graduate Course CHEM-5181: Mass Spectrometry and Chromatography
- The old web page is located here, and I'll be gradually move things over to this Wiki page, which is much easier to update.
Contents
Course Information
- This course WILL be offered in Fall 2011, and WILL NOT be offered in Fall 2012
- Fall 2010 Syllabus
- Registering for the course:
- Every CU student attending the course needs to register, either as (a) for grade; (b) for pass/fail; or (c) for "Audit/No Credit".
- For non-CU students, you can register for the course through the [http://conted.colorado.edu/programs/access/ CU Continuing Education Program ("ACCESS"), again as either (a) for grade; (b) for pass/fail; or (c) for "Audit/No Credit".
- Lectures: Tue & Thu 11:00-12:15, Ekeley W166
- Labs: to be determined, based on the student and MS facility schedules (typically three Tue afternoons 1-3 pm)
- Office hours: immediately after class on Tue & Thu
- Textbooks:
- de Hoffmann & Stroobant. Mass Spectrometry, Principles and Applications, 3rd. Ed. ISBN: 0471485667; Library: QD96.M3 H6413 2007
- McLafferty & Turecek Interpretation of Mass Spectra, 4th Ed., ISBN: 0935702253; Library: QC454.M22 1993;
Lectures
Introduction, Review, Igor
- Introduction and Review of Fundamentals
- Regression Demo Program (PC Download)
- Non-Parametric Regression Demo (Web)
- Least Squares Polynomial Approximation Demo (Web)
- Central Limit Theorem Demo (Web)
- Fourier Synthesis Demo (Web)
- Fourier Analysis Demo (Web)
Mass Spectrometers
- TOFMS
- Mass Spec. Oscilloscope Simulator (PC Download)
Ionization Sources
- Introduction and Electron Ionization
- Ion Optics Simulator
- Prop: EI ionizer, filaments, magnets
- Invited Lecture: the Flowing Afterglow by Prof. Chuck dePuy
- Ionization IV: Bombardment, MALDI and Elemental
- ICP-MS Facility at CU-Boulder
- Prop: MALDI plate
Detectors and Data Acquisition
MS Interpretation
- Introduction to MS Interpretation
- Using Elemental Composition in Interpretation
- IsoPro Isotopic Distribution (PC Download)
- ChemCalc Isotopic Distribution (Online)
- Periodic Table of the Isotopes
- Interpretation of Mass Spectra III (The Molecular Ion)
- Interpretation of Mass Spectra IV (Fragmentation Mechanisms)
Chromatography and Chemical Separations
- Introduction and Theory of Chromatography
- Chromatographic Separation Demo (Web)
- Theory of Chromatography (Cont.)
- Electric-Field Driven Separations: Ion Mobility, Gel Electrophoresis, and Capillary Electrophoresis
- Gas Chromatography
- LC and HPLC
- Ion Chromatography & Thin Layer Chromatography
- Chromatographic Detectors
Homeworks
Reference material for homeworks
HW on Intro and Review of Fundamentals
HW on TOFMS
- HW2: TOFMS
- TOFMS spectrum for Problem #1 (Igor Text File, save and double-click to open in Igor)
HW on Resolution & Accuracy
- HW 3.1: mass spec resolution and accuracy
- Quadrupole MS for donwload (Igor PXP format)
- Orbitrap MS for download (Excel format)
HW on Quadrupoles
- HW3.2: Quadrupole MS simulation
- Understanding the Quadrupole Mass Filter through Computer Simulation, Steel and Henchman, J. Chem. Ed. 75:1049-1054, 1998.
- 4th order Runge-Kutta Integration Method
HW on Ion Optics
- HW4.1: Ion Options Simulation Part 1
- HW4.2: Ion Options Simulation Part 2
- HW4.3: EI Source
- HW4.4: ESI charge states
Labs
Lab 1: MALDI-TOFMS
- catalog.pdf Instrument Description I
- Instrument Description II
- Pre-Lab
- Lab Procedure
- Instrument Instructions