Difference between revisions of "CHEM-5181"
From Jimenez Group Wiki
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* [http://www.colorado.edu/chemistry/masspec/ Home Page of CU Mass Spec. Facility] | * [http://www.colorado.edu/chemistry/masspec/ Home Page of CU Mass Spec. Facility] | ||
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| − | + | === Lab 1: MALDI-TOFMS === | |
| − | + | * [http://www.colorado.edu/chemistry/chem5181/Labs/2010/Voyager_DE_STR catalog.pdf Instrument Description I] | |
| − | + | * [http://www.colorado.edu/chemistry/chem5181/Labs/2010/MALDI_description.pdf Instrument Description II] | |
| − | + | * [http://www.colorado.edu/chemistry/chem5181/Labs/2010/2010_MALDI_Prelab.pdf Pre-Lab] | |
| − | + | * [http://www.colorado.edu/chemistry/chem5181/Labs/2010/2010_MALDI_Lab_Procedure.pdf Lab Procedure] | |
| − | + | * [http://www.colorado.edu/chemistry/chem5181/Labs/2010/MALDI_Instructions_simplified.pdf Instrument Instructions] | |
| − | + | === Lab 2: Electrospray MS === | |
| − | + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/2010_ESI_Prelab.pdf Pre-Lab] | |
| − | + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/2010_ESI_lab.pdf Lab] | |
| − | ** [http:// | + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/Pulsar_description.pdf Pulsar Instrument Description] |
| + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/Pulsar_Instructions_101410.pdf Pulsar Instrument Instructions] | ||
| − | + | === Lab 3: GC-MS of mixtures === | |
| + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/2010_GCMS_prelab1.pdf Pre-Lab] | ||
| + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/2010_GCMS_lab.pdf Lab] | ||
| + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/GCMS_operation1.pdf GC-MS Operation Instructions] | ||
| + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/GC_column_chart1.pdf GC Column Chart] | ||
| + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/GCMS_column_info.pdf GC Column Info] | ||
| + | * [http://cires.colorado.edu/jimenez/CHEM-5181/Labs/ZB_5ms column1.pdf Info on Column used in the lab] | ||
* [http://www.colorado.edu/chemistry/chem5181/Labs/2007_Final_Project.pdf Final Project Organization] | * [http://www.colorado.edu/chemistry/chem5181/Labs/2007_Final_Project.pdf Final Project Organization] | ||
Revision as of 22:55, 5 December 2010
This is the new home for the CU-Boulder Graduate Course CHEM-5181: Mass Spectrometry and Chromatography
- The old web page is located here, and I'll be gradually move things over to this Wiki page, which is much easier to update.
Contents
Course Information
- This course WILL be offered in Fall 2011, and WILL NOT be offered in Fall 2012
- Fall 2010 Syllabus
- Registering for the course:
- Every CU student attending the course needs to register, either as (a) for grade; (b) for pass/fail; or (c) for "Audit/No Credit".
- For non-CU students, you can register for the course through the [http://conted.colorado.edu/programs/access/ CU Continuing Education Program ("ACCESS"), again as either (a) for grade; (b) for pass/fail; or (c) for "Audit/No Credit".
- Lectures: Tue & Thu 11:00-12:15, Ekeley W166
- Labs: to be determined, based on the student and MS facility schedules (typically three Tue afternoons 1-3 pm)
- Office hours: immediately after class on Tue & Thu
- Textbooks:
- de Hoffmann & Stroobant. Mass Spectrometry, Principles and Applications, 3rd. Ed. ISBN: 0471485667; Library: QD96.M3 H6413 2007
- McLafferty & Turecek Interpretation of Mass Spectra, 4th Ed., ISBN: 0935702253; Library: QC454.M22 1993;
Lectures
Introduction, Review, Igor
- Introduction and Review of Fundamentals
- Regression Demo Program (PC Download)
- Non-Parametric Regression Demo (Web)
- Least Squares Polynomial Approximation Demo (Web)
- Central Limit Theorem Demo (Web)
- Fourier Synthesis Demo (Web)
- Fourier Analysis Demo (Web)
Mass Spectrometers
- TOFMS
- Mass Spec. Oscilloscope Simulator (PC Download)
- Quadrupoles
- 3D Ion Traps
Ionization Sources
- Introduction and Electron Ionization
- Ion Optics Simulator
- Prop: EI ionizer, filaments, magnets
- Electrospray and its Ion Chemistry (from Invited Lecturer Dr. Shuji Kato)
- Prop: ESI Source
- Chemical Ionization
- Invited Lecture: the Flowing Afterglow by Prof. Chuck dePuy
- Ionization IV: Bombardment, MALDI and Elemental
- ICP-MS Facility at CU-Boulder
- Prop: MALDI plate
Detectors and Data Acquisition
MS Interpretation
- Introduction to MS Interpretation
- Using Elemental Composition in Interpretation
- IsoPro Isotopic Distribution (PC Download)
- ChemCalc Isotopic Distribution (Online)
- Periodic Table of the Isotopes
- Interpretation of Mass Spectra III (The Molecular Ion)
- Interpretation of Mass Spectra IV (Fragmentation Mechanisms)
Chromatography and Chemical Separations
- Introduction and Theory of Chromatography
- Chromatographic Separation Demo (Web)
- Theory of Chromatography (Cont.)
- Electric-Field Driven Separations: Ion Mobility, Gel Electrophoresis, and Capillary Electrophoresis
- Gas Chromatography
- LC and HPLC
- Ion Chromatography & Thin Layer Chromatography
- Chromatographic Detectors
Homeworks
Reference material for homeworks
HW on Intro and Review of Fundamentals
HW on TOFMS
- HW2: TOFMS
- TOFMS spectrum for Problem #1 (Igor Text File, save and double-click to open in Igor)
HW on Resolution & Accuracy
- HW 3.1: mass spec resolution and accuracy
- Quadrupole MS for donwload (Igor PXP format)
- Orbitrap MS for download (Excel format)
HW on Quadrupoles
- HW3.2: Quadrupole MS simulation
- Understanding the Quadrupole Mass Filter through Computer Simulation, Steel and Henchman, J. Chem. Ed. 75:1049-1054, 1998.
- 4th order Runge-Kutta Integration Method
HW on Ion Optics
- HW4.1: Ion Options Simulation Part 1
- HW4.2: Ion Options Simulation Part 2
- HW4.3: EI Source
- HW4.4: ESI charge states
Labs
Lab 1: MALDI-TOFMS
- catalog.pdf Instrument Description I
- Instrument Description II
- Pre-Lab
- Lab Procedure
- Instrument Instructions
