Difference between revisions of "CHEM-5181"

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*(Ion Trap, Animations) Courtesy of [http://www.thermofinnigan.com Thermo Finnigan]  
 
*(Ion Trap, Animations) Courtesy of [http://www.thermofinnigan.com Thermo Finnigan]  
 
**[http://www.colorado.edu/chemistry/chem5181/IonTrapDemos/1_dampinggas.exe 1: Damping Gas]  
 
**[http://www.colorado.edu/chemistry/chem5181/IonTrapDemos/1_dampinggas.exe 1: Damping Gas]  
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<li><a href="IonTrapDemos/2_trappingmsms.exe">2: Ion Trapping</a>
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**[http://www.colorado.edu/chemistry/chem5181/IonTrapDemos/2_trappingmsms.exe 2: Ion Trapping]
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<li><a href="IonTrapDemos/3_scanning_ms.exe">3: Scanning of the Mass Spectrum</a>
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**[http://www.colorado.edu/chemistry/chem5181/IonTrapDemos/3_scanning_ms.exe 3: Scanning of the Mass Spectrum]
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<li><a href="IonTrapDemos/4_detection_ms.exe">4: Detection of the Mass Spectrum</a>
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**[http://www.colorado.edu/chemistry/chem5181/IonTrapDemos/4_detection_ms.exe 4: Detection of the Mass Spectrum]
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<li><a href="IonTrapDemos/5_esilcq.exe">5: Entire Electrospray + Ion Trap Instrument (Finnigan ESI-LCQ)</a>
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**[http://www.colorado.edu/chemistry/chem5181/IonTrapDemos/5_esilcq.exe">5: Entire Electrospray + Ion Trap Instrument (Finnigan ESI-LCQ)]
−
<li><a href="IonTrapDemos/MSMS_1_elimination.exe">MS/MS 1: Ion Elimination</a> ("There Can Only Be One")
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**[http://www.colorado.edu/chemistry/chem5181/IonTrapDemos/MSMS_1_elimination.exe MS/MS 1: Ion Elimination] ("There Can Only Be One")
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<li><a href="IonTrapDemos/MSMS_2_capture.exe">MS/MS 2: Ion Capture</a>
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**[http://www.colorado.edu/chemistry/chem5181/IonTrapDemos/MSMS_2_capture.exe MS/MS 2: Ion Capture]
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<li><a href="IonTrapDemos/MSMS_3_collision.exe">MS/MS 3: Collision-Induced Dissociation (CID) of the Trapped Ion</a>
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**[http://www.colorado.edu/chemistry/chem5181/IonTrapDemos/MSMS_3_collision.exe MS/MS 3: Collision-Induced Dissociation (CID) of the Trapped Ion]
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</ul>
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*(FTICR, Suggested) [http://pubs.acs.org/cgi-bin/abstract.cgi/ancham/2001/73/i03/abs/ac000973h.html Baseline mass resolution of peptide isobars: A record for molecular mass resolution], He F, Hendrickson CL, Marshall AG, <i>Anal. Chem.</i> 73 (3): 647-650, 2001.
−
<li>(FTICR, Suggested) <a href="http://pubs.acs.org/cgi-bin/abstract.cgi/ancham/2001/73/i03/abs/ac000973h.html">Baseline mass resolution of peptide isobars: A record for molecular mass resolution</a>, He F, Hendrickson CL, Marshall AG, <i>Anal. Chem.</i> 73 (3): 647-650, 2001.
+
*(FTICR, Suggested) [http://www3.interscience.wiley.com/cgi-bin/abstract/24610/ABSTRACT Fourier Transform Mass Spectrometry (Tutorial)] I. J. Amster, JMS, 1996, 1325-1337.  
−
<li>(FTICR, Suggested) <a href="http://www3.interscience.wiley.com/cgi-bin/abstract/24610/ABSTRACT">Fourier Transform Mass Spectrometry (Tutorial)</a> I. J. Amster, JMS, 1996, 1325-1337.  
+
*(FTICR, Advanced) [http://www3.interscience.wiley.com/cgi-bin/abstract/10009487/ABSTRACT Fourier transform ion cyclotron resonance mass spectrometry: A primer]. A.G. Marshall et al., <i>Mass Spec. Rev.</i> 17: 1-35, 1998.
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<li>(FTICR, Advanced) <a href="http://www3.interscience.wiley.com/cgi-bin/abstract/10009487/ABSTRACT">Fourier transform ion cyclotron resonance mass spectrometry: A primer</a>. A.G. Marshall et al., <i>Mass Spec. Rev.</i> 17: 1-35, 1998.
+
*(Orbitrap, Advanced) [http://www3.interscience.wiley.com/cgi-bin/abstract/110471441/ABSTRACT The Orbitrap: a new mass spectrometer (Perspective)]. R.G. Cooks et al., <i>J Mass Spec.</i> 40: 430-443, 2005.
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<li>(Orbitrap, Advanced) <a href="http://www3.interscience.wiley.com/cgi-bin/abstract/110471441/ABSTRACT">The Orbitrap: a new mass spectrometer (Perspective)</a>. R.G. Cooks et al., <i>J Mass Spec.</i> 40: 430-443, 2005.
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*(Q-TOF, Advanced) [http://www3.interscience.wiley.com/cgi-bin/abstract/85010411/ABSTRACT An introduction to quadrupole–time-of-flight mass spectrometry] I. V. Chernushevich, A. V. Loboda, and B. A. Thomson, JMS, 2001, 849-865
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<li> (Q-TOF, Advanced) <a href = "http://www3.interscience.wiley.com/cgi-bin/abstract/85010411/ABSTRACT"> An introduction to quadrupole–time-of-flight mass spectrometry </a> I. V. Chernushevich, A. V. Loboda, and B. A. Thomson, JMS, 2001, 849-865
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*(MS/MS, Suggested) [http://www.hopkinsmedicine.org/mams/MAMS/middleframe_files/teaching_files/ME330.884/2005/MS2005-Lecture-8-Tandems-and-Hybrids.pdf Lecture on MS/MS from Prof. Bob Cotter]
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<li>(MS/MS, Suggested) <a href="http://www.hopkinsmedicine.org/mams/MAMS/middleframe_files/teaching_files/ME330.884/2005/MS2005-Lecture-8-Tandems-and-Hybrids.pdf">Lecture on MS/MS from Prof. Bob Cotter</a>
+
*(MCP, Advanced) [http://www.burle.com/cgi-bin/byteserver.pl/pdf/tp214.pdf Microchannel Plate Detectors] J. L. Wiza, Nuc Inst Meth, 1979, 587-601.
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<li>(MCP, Advanced) <a href="http://www.burle.com/cgi-bin/byteserver.pl/pdf/tp214.pdf">Microchannel Plate Detectors</a> J. L. Wiza, Nuc Inst Meth, 1979, 587-601.
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*(Aerosol, Suggested)[http://www3.interscience.wiley.com/cgi-bin/abstract/72001802/ABSTRACT?CRETRY=1&SRETRY=0 Tutorial: Sampling and analysis of individual particles by aerosol mass spectrometry.], M.V. Johnston, <i>J. Mass Spectrom.</i> 35, 585–595 (2000).
−
<li>(Aerosol, Suggested)<a href="http://www3.interscience.wiley.com/cgi-bin/abstract/72001802/ABSTRACT?CRETRY=1&SRETRY=0">Tutorial: Sampling and analysis of individual particles by aerosol mass spectrometry. </a>, M.V. Johnston, <i>J. Mass Spectrom.</i> 35, 585–595 (2000).
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*(Aerosol, Suggested) Chemical and Microphysical Characterization of Ambient Aerosols with the Aerodyne Aerosol Mass Spectrometer. Canagaratna et al., <i>Mass Spectrometry Reviews</i>, in press, April 2006. [http://www.colorado.edu/chemistry/chem5181/Papers/MSReview_Final.pdf PDF of accepted version] and [http://www.colorado.edu/chemistry/chem5181/Papers/MSReview_Figures.pdf Figures]
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<li>(Aerosol, Suggested) Chemical and Microphysical Characterization of Ambient Aerosols with the Aerodyne Aerosol Mass Spectrometer. Canagaratna et al., <i>Mass Spectrometry Reviews</i>, in press, April 2006. <a href="Papers/MSReview_Final.pdf">PDF of accepted version</a> and <a href="Papers/MSReview_Figures.pdf">Figures</a>
+
*(Aerosol, Advanced) [http://www.aaar.org/ast_abst/v33n12pref.htm Special Issue of AS&T on Aerosol Mass Spectrometry]
−
<li>(Aerosol, Advanced) <a href="http://www.aaar.org/ast_abst/v33n12pref.htm">Special Issue of AS&T on Aerosol Mass Spectrometry</a></li>
+
*(Aerosol, Link to references) [http://cires.colorado.edu/jimenez/ams-papers.html Publications using the Aerodyne Aerosol Mass Spectrometer (AMS)]
−
<li>(Aerosol, Link to references) <a href="http://cires.colorado.edu/jimenez/ams-papers.html">Publications using the Aerodyne Aerosol Mass Spectrometer (AMS)</a>
+
*(Aerosol, Link to references) [http://cires.colorado.edu/jimenez/ams.html Aerosol Mass Spectrometry Web Page]
−
<li>(Aerosol, Link to references) <a href="http://cires.colorado.edu/jimenez/ams.html">Aerosol Mass Spectrometry Web Page</a>
+
*(Aerosol, Link to references) [http://mae.ucdavis.edu/wexler/spa_refs.html A List of Publications in Single Particle Mass Spectrometry], maintained by Prof. Tony Wexler.
−
<li>(Aerosol, Link to references) <a href="http://mae.ucdavis.edu/wexler/spa_refs.html">A List of Publications in Single Particle Mass Spectrometry</a>, maintained by Prof. Tony Wexler.
+
*(Aerosol, Link to references) [http://www.udel.edu/chem/johnston/SPMSdbase.html Another List of Publications in Single Particle Mass Spectrometry], this one maintained by Prof. Murray Johnston.
−
<li>(Aerosol, Link to references) <a href="http://www.udel.edu/chem/johnston/SPMSdbase.html">Another List of Publications in Single Particle Mass Spectrometry</a>, this one maintained by Prof. Murray Johnston.
 

Revision as of 14:42, 16 December 2010

This is the new home for the CU-Boulder Graduate Course CHEM-5181: Mass Spectrometry and Chromatography

  • The old web page is located here, and I'll be gradually move things over to this Wiki page, which is much easier to update.

Course Information

  • This course WILL be offered in Fall 2011, and WILL NOT be offered in Fall 2012
  • Fall 2010 Syllabus
  • Registering for the course:
    • Every CU student attending the course needs to register, either as (a) for grade; (b) for pass/fail; or (c) for "Audit/No Credit".
    • For non-CU students, you can register for the course through the CU Continuing Education Program ("ACCESS"), again as either (a) for grade; (b) for pass/fail; or (c) for "Audit/No Credit".
  • Lectures: Tue & Thu 11:00-12:15, Ekeley W166
  • Labs: to be determined, based on the student and MS facility schedules (typically three Tue afternoons 1-3 pm)
  • Office hours: immediately after class on Tue & Thu
  • Textbooks:
    • de Hoffmann & Stroobant. Mass Spectrometry, Principles and Applications, 3rd. Ed. ISBN: 0471485667; Library: QD96.M3 H6413 2007
    • McLafferty & Turecek Interpretation of Mass Spectra, 4th Ed., ISBN: 0935702253; Library: QC454.M22 1993;

FAQs about CHEM-5181

  • Can I audit the class?
    • Answer: Yes, but you need to register for it as "Audit / No Credit", either as a regular CU student or through the ACCESS Program. Auditors typically attend lectures but do not do the labs, homeworks, or exams, unless they want to.
  • But I don't want to register at all. Can I still audit the class?
    • Answer: No.
  • I work in industry and use MS and chromatography in my work, can I take the class?
    • Answer: Yes, registering through the ACCESS Program. Some things to think about: (a) you need to have the relevant background or be willing to work to gain the background, especially if you want to take the class for credit. (b) This is a class for 1st year graduate students in Anaytical & Environmental Chemistry, and it takes quite a bit of work to take the class for credit. If you don't have much time for the class due to your work responsibilities, you should register as "Audit / No Credit".

Lectures

Introduction, Review, Igor

Mass Spectrometers

  • 3D Ion Traps
    • Required Reading: de Hoffmann and Stroobant 2007, Section 2.2

Ionization Sources

MS Detectors, Data Acquisition, Vacuum

  • Vacuum Technology
    • Props: turbo pump, diaphragm pump

MS Interpretation

  • Interpretation of Mass Spectra III (The Molecular Ion)
    • Required Reading: McLafferty and Turecek 1993, Chapters 3 and 5
  • Interpretation of Mass Spectra IV (Fragmentation Mechanisms)
    • Required Reading: McLafferty and Turecek 1993, Chapters 4, and skim 9

Chromatography and Chemical Separations

  • Gas Chromatography
    • Prop: GC column
  • LC and HPLC
  • Ion Chromatography & Thin Layer Chromatography
  • Chromatographic Detectors

Homeworks

Reference material for homeworks

HW on Intro and Review of Fundamentals

HW on TOFMS

HW on Resolution & Accuracy

HW on Quadrupoles

HW on Ion Optics

Labs

Lab 1: MALDI-TOFMS

Lab 2: Electrospray MS

Lab 3: GC-MS of mixtures

Reference Materials

Suggestions Encouraged. Please send links to jose.jimenez at colorado.edu (All .pdf's are password restricted)

Introduction to Mass Spectrometry

Ionization Methods

Mass Analyzers

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